trypan blue was used to differentiate dead cells.
isolated whole blood leukocytes
live cells= 24
dead cell=11
viability=63%
mononuclear cells (PBMC)
live cells= 297
dead cell=84
viability=77.95%
the cell number significantly increased? why this might be. this value was expected or not.
why an increase in trypan blue stained (dead) cells?
